Native Mass Spectrometry: Recent Progress and Remaining Challenges
Related Experiment Video
Updated: Oct 8, 2025

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
Published on: October 15, 2018
Kelly R Karch1,2, Dalton T Snyder2, Sophie R Harvey1,2
1Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA;
Abstract:
Native mass spectrometry (nMS) has emerged as an important tool in studying the structure and function of macromolecules and their complexes in the gas phase. In this review, we cover recent advances in nMS and related techniques including sample preparation, instrumentation, activation methods, and data analysis software. These advances have enabled nMS-based techniques to address a variety of challenging questions in structural biology. The second half of this review highlights recent applications of these technologies and surveys the classes of complexes that can be studied with nMS. Complementarity of nMS to existing structural biology techniques and current challenges in nMS are also addressed.
More Related Videos
Related Concept Videos
Mass Spectrometry: Overview
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Mass Spectrometers
Mass Analyzers: Overview
Mass Spectrometry: Isotope Effect
High-Resolution Mass Spectrometry (HRMS)

