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Identification of ceRNA (lncRNA-miRNA-mRNA) Regulatory Network in Myocardial Fibrosis After Acute Myocardial
Shuo Wang1, Yuying Liu1, Xitian Hu1
1Department of Cardiovasology, Shijiazhuang People's Hospital, Shijiazhuang, People's Republic of China.
Insights
Researchers identified key biomarkers for myocardial fibrosis (MF) after acute myocardial infarction (AMI). These findings may help predict heart failure development following AMI.
Area of Science:
- Biomedical research
- Molecular biology
- Cardiovascular science
Background:
- Myocardial fibrosis (MF) following acute myocardial infarction (AMI) is a significant precursor to heart failure.
- Identifying reliable biomarkers for MF post-AMI is crucial for clinical intervention and patient outcomes.
Purpose of the Study:
- To identify critical biomarkers associated with myocardial fibrosis after acute myocardial infarction.
- To explore potential molecular interactions underlying MF development post-AMI.
Main Methods:
- RNA sequencing was employed to identify differentially expressed mRNAs, miRNAs, and lncRNAs in AMI and MF samples.
- A competing endogenous RNA (ceRNA) network was constructed to analyze interactions between these molecules.
- Quantitative real-time PCR (qRT-PCR) was used for validation of candidate biomarkers.
Main Results:
- Numerous differentially expressed RNAs (DEmRNAs, DEmiRNAs, DElncRNAs) were identified.
- A ceRNA network comprising 9 lncRNA-miRNA and 9 miRNA-mRNA pairs was established.
- Expression patterns of most candidate biomarkers were validated by qRT-PCR, with minor exceptions.
Conclusions:
- The study proposes LINC00664/hsa-miR-197-3p/JAK2 and GAS6-AS1/SNHG22/hsa-miR-135a-5p/CENPB/BCL9L interaction pairs as potential biomarkers for MF post-AMI.
- These identified molecular pathways offer insights into the mechanisms of MF development.
- Further research is warranted to validate these biomarkers in clinical settings.
Purpose:
Myocardial fibrosis (MF) after acute myocardial infarction (AMI) ultimately results in heart failure, which is a serious threat to human life. This study aimed to excavate critical biomarkers associated with MF after AMI.
Materials And Methods:
RNA-sequencing was performed to obtain differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs) and lncRNAs (DElncRNAs) in AMI and MF after AMI.
Results:
Abundant DEmRNAs, DEmiRNAs and DElncRNAs were identified in AMI and MF after AMI. The ceRNA network, which contained 9 lncRNA-miRNA pairs and 9 miRNA-mRNA pairs, was acquired. In AMI, all candidate markers generally exhibited the same pattern as that in our RNA-seq results; while in MF after AMI, except for CENPB, JAK2 and hsa-miR-197-3p, the expression of the others in the qRT-PCR results exhibited the same pattern as that in our RNA-seq results.
Conclusion:
We speculated that LINC00664/hsa-miR-197-3p/JAK2 and GAS6-AS1/SNHG22/hsa-miR-135a-5p/CENPB/BCL9L interaction pairs may serve as potential biomarkers in MF after AMI.
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