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Related Experiment Video

Updated: Oct 7, 2025

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Published on: March 6, 2015

Sodium/hydrogen-exchanger-2 modulates colonocyte lineage differentiation.

Katerina Nikolovska¹, Li Cao^1,2, Inga Hensel¹

¹Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany.

Acta Physiologica (Oxford, England)

|January 5, 2022

Summary

Sodium/hydrogen exchanger 2 (NHE2) activity supports absorptive enterocyte differentiation in the colon. NHE2 activation in progenitor cells regulates intracellular pH, influencing cell development and function.

Keywords:

NHE2 colonoids intestinal differentiation intestinal electrolyte transport pHi regulation sodium/hydrogen exchange

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Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Published on: March 30, 2015

Area of Science:

Gastroenterology
Cell Biology
Molecular Biology

Background:

The sodium/hydrogen exchanger 2 (NHE2) is an acid extruder in the intestine with a poorly understood physiological role.
Its localization in colonic crypts suggests involvement in epithelial cell dynamics.

Purpose of the Study:

To investigate the function of NHE2 in colonic epithelial cell proliferation, differentiation, and electrolyte transport.
To elucidate the physiological significance of NHE2 in the colon.

Main Methods:

Utilized murine nhe2 knockout (nhe2-/-) and wild-type (nhe2+/+) colonic crypts and colonoids.
Employed pH-fluorometry, gene expression analysis, and immunofluorescence in cell models.

Main Results:

NHE2 deficiency led to decreased intracellular pH in colonic crypts.
NHE2 expression increased in progenitor cells, supporting absorptive enterocyte differentiation.
Absence of NHE2 altered differentiation markers, favoring secretory over absorptive lineages.

Conclusions:

NHE2 is activated as colonocytes exit the stem cell niche.
NHE2 activity is crucial for regulating progenitor cell pH and promoting absorptive enterocyte differentiation.