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Related Experiment Videos

The ischaemic leg as a source of complement activation.

A Bengtson1, P Holmberg, M Heideman

  • 1Department of Anaesthesiology and Intensive Care, Sahlgren's Hospital, University of Gothenburg, Sweden.

The British Journal of Surgery
|August 1, 1987
PubMed
Summary

This study shows that complement activation, measured by anaphylatoxins (C3a and C5a), is elevated in patients with ischemic legs before surgery. Levels normalize within a week, with acute ischemia showing higher activation.

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Area of Science:

  • Immunology
  • Vascular Surgery
  • Complement System

Background:

  • The complement system plays a crucial role in immune responses.
  • Ischemic limb disease can lead to systemic complications.
  • Anaphylatoxins are key mediators of complement activation.

Purpose of the Study:

  • To investigate complement activation in patients undergoing surgical resection for ischemic legs.
  • To compare complement activation levels in acute versus chronic ischemia.
  • To assess changes in anaphylatoxin levels and inhibitor activity pre- and post-operatively.

Main Methods:

  • Studied 40 patients with ischemic legs (17 acute, 23 chronic).
  • Measured plasma anaphylatoxin (C3a, C5a) concentrations and anaphylatoxin inhibitor (AI) activity.

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  • Collected samples pre-operatively and at 1 hour, 24 hours, and 1 week post-operatively.

    • Main Results:

    • Elevated plasma anaphylatoxin concentrations were observed pre-operatively.
    • Anaphylatoxin levels returned to normal by one week post-surgery.
    • Patients with acute ischemia exhibited higher C3a and C5a levels pre-operatively and during the first 24 hours post-operatively compared to those with chronic ischemia.
    • Anaphylatoxin activity may contribute to pre-operative organ insufficiency in extensive ischemia.

    Conclusions:

    • Complement activation is significantly increased in patients with ischemic legs prior to surgical intervention.
    • Acute ischemia is associated with a more pronounced complement activation response.
    • These findings suggest a potential role for complement-mediated inflammation in the pathophysiology of ischemic limb disease and associated organ dysfunction.