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Identification and complete genome sequence of mulberry cryptic virus 1
Jinhong Yang1, Yunwu Peng1, Weiqing Kong2
1Shaanxi Key Laboratory of Sericulture, Ankang University, Shaanxi, 725099, China.
Archives of Virology
|January 5, 2022
Summary
A novel cryptic virus, mulberry cryptic virus 1 (MuCV1), was discovered in mulberry plants. Phylogenetic analysis confirms MuCV1 belongs to the deltapartitivirus genus, expanding our understanding of plant virology.
Area of Science:
- Plant Virology
- Molecular Biology
- Bioinformatics
Background:
- Cryptic viruses are challenging to detect and characterize.
- Mulberry (Morus alba) is an economically important plant species.
- Understanding viral diversity in crops is crucial for agricultural health.
Purpose of the Study:
- To identify and characterize a novel cryptic virus in mulberry.
- To determine the genomic structure and phylogenetic placement of the new virus.
Main Methods:
- Transcriptome sequencing of mulberry samples.
- Rapid amplification of cDNA ends (RACE) for genome confirmation.
- Sequence analysis and phylogenetic reconstruction using RdRp and CP genes.
Main Results:
- Identification of mulberry cryptic virus 1 (MuCV1).
- MuCV1 genome consists of two double-stranded RNAs encoding RdRp and CP.
- Phylogenetic analysis places MuCV1 within the Deltapartitivirus genus.
Conclusions:
- MuCV1 represents a newly discovered deltapartitivirus in mulberry.
- The conserved 5'-AGAAUU-3' sequence may play a role in viral replication.
- This finding contributes to the knowledge of cryptic viruses in Rosaceae family plants.
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