Related Experiment Video
Updated: Oct 7, 2025
Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
Published on: October 12, 2018
Ex Vivo HIV Infection Model of Cervico-Vaginal and Rectal Tissue
Louise A Ouattara1, Nikolas C Vann2, Gustavo F Doncel2
1CONRAD, Eastern Virginia Medical School, Norfolk, VA, USA. yaoal@evms.edu.
Abstract:
Globally, the most frequent route of HIV transmission is through sexual intercourse. In women, sexual transmission of HIV involves cervical, vaginal, endometrial, and rectal mucosal exposure to the virus. Here we describe technical protocols for ex vivo cervical, vaginal, and rectal tissue infection models and cultures that can be used to assess tissue susceptibility to infection under different conditions as well as the potential antiviral efficacy of a treatment for HIV prevention or cure.
