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Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus.

Orion M Venero1, Wakana Sato1, Joseph M Heili1

  • 1Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 5, 2022
PubMed
Summary

This study introduces a novel 3D-printable microfluidics device for simultaneous liposome formation and encapsulation. This method efficiently creates biomimetic synthetic cells with diverse internal chemistries.

Keywords:
3D-printableGiant unilamellar vesiclesLiposomal encapsulationMicrofluidicsSynthetic cells

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Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Materials Science

Background:

  • Liposomes are crucial for engineering biomimetic and synthetic cells.
  • Conventional methods like thin film rehydration (TFH) and reverse emulsion encapsulation (REE) have limitations.
  • Microfluidics offers advanced control but often focuses on lumen encapsulation separately from liposome formation.

Purpose of the Study:

  • To develop a novel microfluidics device for simultaneous liposome formation and encapsulation.
  • To enable the creation of synthetic cells with diverse, cell-mimetic lumen chemistries.
  • To improve the efficiency and versatility of liposome preparation for synthetic cell engineering.

Main Methods:

  • Utilized a 3D-printable microcapillary-based microfluidics device.
  • Adapted the droplet-shooting and size-filtration (DSSF) liposome preparation method.
  • Achieved simultaneous formation of liposomes and encapsulation of lumen contents.

Main Results:

  • Successfully demonstrated simultaneous liposome formation and encapsulation.
  • Enabled encapsulation of various cell-mimetic lumen chemistries within liposomes.
  • The developed device offers a streamlined approach for synthetic cell construction.

Conclusions:

  • The 3D-printable microfluidics device provides a powerful new tool for synthetic cell engineering.
  • This method facilitates the creation of complex biomimetic systems.
  • It advances the field of liposome-based synthetic cell development.