Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proton (¹H) NMR: Chemical Shift01:07

Proton (¹H) NMR: Chemical Shift

2.1K
Organic molecules primarily contain carbon and hydrogen atoms. While all the hydrogen isotopes are NMR-active, protium or hydrogen-1 is the most abundant. It has a significant energy separation between its nuclear spin states due to its large gyromagnetic ratio. As per Boltzmann's distribution, an increase in the energy separation implies a greater excess population of nuclei available for excitation, resulting in a strong NMR absorption signal.
Absorption signals of all the protium nuclei...
2.1K
Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule01:10

Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule

1.5K
In the AX proton spin system, proton A can sense the two spin states of a coupled proton X, resulting in a doublet NMR signal with two peaks of equal (1:1) intensity. When proton A is coupled to two equivalent protons (AX2 spin system), the spin states of each X can be aligned with or against the external field, creating three possible scenarios. This results in a 1:2:1  triplet signal, where the central peak corresponds to the chemical shift of A and is twice as large or intense as the...
1.5K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

341
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
341
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

1.4K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
1.4K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

584
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
584
¹H NMR of Labile Protons: Temporal Resolution01:10

¹H NMR of Labile Protons: Temporal Resolution

1.3K
Protons bonded to heteroatoms such as nitrogen and oxygen exhibit a range of chemical shift values. This is due to the varying degree of hydrogen bonding between the proton and the heteroatom in other molecules. The extent of hydrogen bonding affects the electron density around the proton, thereby giving different chemical shift values for the protons in the proton NMR spectrum.
The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the specific...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Green, Facile Method for Selective α‑Bromination of Flavanones.

ACS omega·2026
Same author

Understanding the Effect of Surface-Grafted Polyethylene Glycol Chains on Scavenger Endothelial Cell Sequestration of Polymeric Nanoparticles via Quantitative Pharmacokinetic Analysis in Zebrafish Larvae.

ACS applied materials & interfaces·2025
Same author

From molecular descriptions to cellular functions of intrinsically disordered protein regions.

Biophysics reviews·2024
Same author

Preferential solvation of carbohydrates in water-trifluoroethanol mixtures: a solvent detected heteronuclear NMR approach.

Physical chemistry chemical physics : PCCP·2021
Same author

Assessment of the Role of 2,2,2-Trifluoroethanol Solvent Dynamics in Inducing Conformational Transitions in Melittin: An Approach with Solvent <sup>19</sup>F Low-Field NMR Relaxation and Overhauser Dynamic Nuclear Polarization Studies.

The journal of physical chemistry. B·2020
Same author

Correction: Conformation of bis-nitroxide polarizing agents by multi-frequency EPR spectroscopy.

Physical chemistry chemical physics : PCCP·2020
Same journal

Photoinduced Charge-Transfer Suppresses Triplet Formation Efficiency in Thiocoumarins: Evidence from Ultrafast Spectroscopy and Theoretical Calculations.

The journal of physical chemistry. A·2026
Same journal

Porphyrin Aggregation Revisited: From the Four-Orbital Gouterman Model to an Eight-Orbital Framework in Porphin H-Dimers.

The journal of physical chemistry. A·2026
Same journal

Unraveling the Electronic Origin of Selectivity in Ambimodal Transition States with Valence Bond Theory.

The journal of physical chemistry. A·2026
Same journal

Mechanism and Kinetics of the Initial Oxidative Ring-Opening of Corannulene Radicals under Combustion Conditions.

The journal of physical chemistry. A·2026
Same journal

High-Resolution Absorption Spectroscopy of ND<sub>3</sub> between 59,000 and 93,000 cm<sup>-1</sup>.

The journal of physical chemistry. A·2026
Same journal

Twisted-Driven Photoionization of Aligned Chiral Molecules: Signatures of Circular and Helical Dichroism.

The journal of physical chemistry. A·2026
See all related articles

Related Experiment Video

Updated: Oct 7, 2025

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.2K

1H NMR with Partial Transition Selectivity.

Christy George1, N Chandrakumar2

  • 1Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.

The Journal of Physical Chemistry. A
|January 5, 2022
PubMed
Summary
This summary is machine-generated.

A new CArbon Single transition EDited (CASED) 1H NMR technique simplifies complex spectra by selectively editing proton signals. This method reduces spectral crowding while preserving crucial information about proton-proton couplings.

More Related Videos

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

1.9K
Spin Saturation Transfer Difference NMR SSTD NMR: A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes
11:44

Spin Saturation Transfer Difference NMR SSTD NMR: A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes

Published on: November 12, 2016

18.1K

Related Experiment Videos

Last Updated: Oct 7, 2025

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.2K
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

1.9K
Spin Saturation Transfer Difference NMR SSTD NMR: A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes
11:44

Spin Saturation Transfer Difference NMR SSTD NMR: A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes

Published on: November 12, 2016

18.1K

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Organic Chemistry
  • Analytical Chemistry

Background:

  • Proton NMR (1H NMR) is highly sensitive but suffers from spectral crowding due to limited chemical shift ranges.
  • Existing methods like broadband decoupling simplify spectra but sacrifice coupling information.
  • Resolving complex 1H NMR spectra is crucial for molecular structure elucidation.

Purpose of the Study:

  • To introduce a novel 1D NMR experiment for simplifying 1H NMR spectra.
  • To reduce spectral crowding while retaining information on homonuclear scalar couplings.
  • To enable easier assignment and interpretation of complex 1H NMR spectra.

Main Methods:

  • Development of a novel one-dimensional (1D) NMR experiment termed CArbon Single transition EDited (CASED) 1H NMR.
  • The experiment utilizes two-way coherence transfer from 1H to 13C and back to 1H detection.
  • Natural abundance 13C nuclei are employed in the coherence transfer pathway.

Main Results:

  • The CASED 1H NMR experiment achieves partial 1H multiplet selectivity, reducing spectral crowding.
  • The method allows for the inference of homonuclear scalar couplings, unlike broadband decoupling.
  • Unusual spectral patterns were observed, summarized, and rationalized for various molecular fragments.
  • Artifacts inherent to the current CASED version were identified and described.

Conclusions:

  • CASED 1H NMR offers a valuable new approach to simplify complex proton NMR spectra.
  • The technique successfully balances spectral simplification with the retention of coupling information.
  • Demonstrated application on a disaccharide showcases the practical utility of CASED 1H NMR for structural analysis.