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Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
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Tools for Detecting a "Superbug": Updates on Candida auris Testing.

Shawn R Lockhart1, Meghan M Lyman1, D Joseph Sexton1

  • 1Mycotic Diseases Branch, Centers for Disease Control and Preventiongrid.416738.f, Atlanta, Georgia, USA.

Journal of Clinical Microbiology
|January 5, 2022
PubMed
Summary
This summary is machine-generated.

Candida auris, a yeast causing difficult-to-treat infections, is increasingly detected using improved methods. Advances in diagnostics like MALDI-TOF MS and specialized CHROMagar now aid in identifying this rapidly spreading, antifungal-resistant pathogen.

Keywords:
CHROMagarCandida aurisantifungal susceptibility testingfungaltesting

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Area of Science:

  • Medical Mycology
  • Clinical Microbiology
  • Infectious Diseases

Background:

  • Candida auris is an emerging fungal pathogen known for healthcare-associated transmission.
  • This yeast exhibits rapid development of antifungal resistance, complicating treatment.
  • Early detection was limited to DNA sequencing, posing challenges for clinical laboratories.

Purpose of the Study:

  • To review current diagnostic tools and resources for Candida auris identification.
  • To highlight advancements in Candida auris detection methods over the past decade.

Main Methods:

  • Review of diagnostic technologies including matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS).
  • Assessment of laboratory-developed tests and commercial kits for Candida auris detection.
  • Evaluation of specialized CHROMagar media for yeast identification.

Main Results:

  • MALDI-TOF MS databases have been expanded to include Candida auris.
  • Both in-house and commercial diagnostic kits are now available.
  • Specialized CHROMagar media facilitate laboratory identification.

Conclusions:

  • Significant progress has been made in the detection and identification of Candida auris.
  • Current tools offer improved capabilities for clinical microbiology laboratories.
  • Enhanced diagnostic resources are crucial for managing Candida auris outbreaks.