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Influencing elements of codon usage bias in Birnaviridae and its evolutionary analysis
Nazrana Sultana Begum1, Supriyo Chakraborty1
1Department of Biotechnology, Assam University, Silchar 788011, Assam, India.
Birnaviridae viruses exhibit low codon usage bias (CUB), influenced by both mutation and natural selection. Natural selection is the primary driver shaping CUB in these double-stranded RNA viruses.
Area of Science:
- Virology
- Genomics
- Bioinformatics
Background:
- Birnaviridae are double-stranded RNA viruses causing significant economic losses in fish and poultry.
- Understanding viral genome properties is crucial for disease control and evolutionary research.
Purpose of the Study:
- To investigate the codon usage bias (CUB) patterns in the Birnaviridae family.
- To determine the factors influencing CUB in these viruses.
Main Methods:
- Bioinformatic analysis of Birnaviridae coding sequences.
- Evaluation of parameters including ENC, RSCU, nucleotide skewness, and neutrality plots.
Main Results:
- Birnaviridae showed low CUB with high GC content.
- Both mutational pressure and natural selection contribute to CUB.
- Natural selection was identified as the dominant factor shaping CUB.
Conclusions:
- Codon usage in Birnaviridae is primarily shaped by natural selection.
- This study provides foundational data for evolutionary research on Birnaviridae.
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