Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>OCA2</i> common variant NM_000275.3:c.574-19A>G affects splicing and is pathogenic.

Molecular genetics and metabolism reports·2026
Same author

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same author

Postpartum delirious mania in a patient with diagnosis of bipolar disorder after cesarean delivery: a case report.

Frontiers in psychiatry·2026
Same author

Molecular detection and subtype characterization of Blastocystis in diarrheal outpatients in Shanghai, China: A case-control study.

PLoS neglected tropical diseases·2026
Same author

DirectASRM: uncovering allele-specific post-transcriptional RNA modifications through direct RNA sequencing.

Bioinformatics (Oxford, England)·2026
Same author

Molecular Basis of Synergistic Tau Amyloid Core Inhibition by K311/K353 Acetylation via Microsecond MD Simulations.

The journal of physical chemistry. B·2026

Related Experiment Video

Updated: Oct 7, 2025

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.9K

m5C-Atlas: a comprehensive database for decoding and annotating the 5-methylcytosine (m5C) epitranscriptome.

Jiongming Ma1,2, Bowen Song3,4, Zhen Wei2,5

  • 1Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350004, China.

Nucleic Acids Research
|January 6, 2022
PubMed
Summary

m5C-Atlas is a new database that collects and annotates RNA 5-methylcytosine (m5C) sites across 13 species. This resource provides comprehensive data on m5C modifications, aiding epitranscriptome research.

More Related Videos

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.4K
Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study
06:57

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study

Published on: July 7, 2023

1.3K

Related Experiment Videos

Last Updated: Oct 7, 2025

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.9K
Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.4K
Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study
06:57

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study

Published on: July 7, 2023

1.3K

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • 5-Methylcytosine (m5C) is a prevalent RNA modification regulating gene expression.
  • Understanding m5C's role in RNA functions like stability and translation is crucial.
  • Existing resources lack comprehensive, integrated data on m5C sites.

Purpose of the Study:

  • To develop m5C-Atlas, a centralized database for RNA 5-methylcytosine (m5C) sites.
  • To provide comprehensive annotation and analysis of m5C modifications across species.
  • To facilitate exploration of m5C epitranscriptomes and their regulatory roles.

Main Methods:

  • Collected and curated 166,540 m5C sites from 13 species using 5 epitranscriptome profiling technologies.
  • Quantified condition-specific methylation levels from 351 RNA bisulfite sequencing samples.
  • Developed an integrative pipeline for data analysis and annotation.

Main Results:

  • m5C-Atlas integrates data from diverse sources, offering base-resolution m5C site information.
  • The database includes novel features: evolutionary conservation, SNP association, and RNA secondary structure relevance.
  • Condition-specific methylation levels are available for 351 samples from 22 studies.

Conclusions:

  • m5C-Atlas provides a user-friendly platform for exploring and annotating m5C epitranscriptomes.
  • The database enables deeper investigation into post-transcriptional mechanisms involving m5C.
  • This resource offers unprecedented opportunities for advancing epitranscriptomics research.