Related Experiment Video

Updated: Jun 23, 2026

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
18:45

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

Published on: September 2, 2012

Author Correction: Cycloalkane-modified amphiphilic polymers provide direct extraction of membrane proteins for

Anna J Higgins1, Alex J Flynn1, Anaïs Marconnet2,3

  • 1School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK.

Communications Biology
|January 6, 2022
PubMed
Abstract

No abstract available in PubMed .

More Related Videos

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
11:45

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

Published on: February 27, 2020

Related Experiment Videos

Last Updated: Jun 23, 2026

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
18:45

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

Published on: September 2, 2012

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
11:45

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

Published on: February 27, 2020

Related Concept Videos

Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

5.5K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
5.5K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.7K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.7K

Articles linked to this work by shared authors, journal, and citation graph.

Direct deposition on pre-cooled grids: a new approach to sub-millisecond cryoEM grid preparation.

Acta crystallographica. Section D, Structural biology·2026

Towards light-coupled sample preparation for time-resolved cryoEM studies.

IUCrJ·2026

CryoEM structures reveal allosteric regulation of the catalytic activity of the multi-protein human MAT enzyme complexes.

IUCrJ·2026

Cross-Platform Assessment of Sub-50 nm Nanopipette Emitters for Native Electrospray Ionization Mass Spectrometry.

bioRxiv : the preprint server for biology·2026

Nanopipettes Enable Native Mass Spectrometry Studies of the Intrinsically Disordered Protein α-Synuclein in Biochemical Buffers.

Analytical chemistry·2026

Recommendations and considerations for hydroxyl radical protein footprinting-mass spectrometry.

Nature methods·2026

A who's who of cell types in skeletal muscle.

Communications biology·2026

On variability in local field potentials.

Communications biology·2026

A Single-organoid Workflow for quantitative Imaging classiFication and Tracking (SWIFT).

Communications biology·2026

SARS-CoV-2 Delta variant-specific ORF3a mutations destabilize lysosomal homeostasis to trigger lysosomal damage-mediated cell death.

Communications biology·2026

Self-supervised learning yields representational signatures of category-selective cortex.

Communications biology·2026

Genetic transformation and CRISPR/Cas12a-mediated gene editing of European beech (Fagus sylvatica L.) employing a transient protoplast system.

Communications biology·2026

Studying Mucin-Derived O-Glycopeptides with Gas-Phase FRET.

Analytical chemistry·2026

Structural insights into RNA phosphorylation by the RNase PNK module of the human rixosome complex.

Nucleic acids research·2026

Catalytic [6 + 3] cycloaddition for the synthesis of medium-sized ring systems: a critical review.

RSC advances·2026

Single-Molecule Detection of Enzymatic PET Hydrolysis Using Fluorogenic Probe-Doped PET Nanoparticles.

Small (Weinheim an der Bergstrasse, Germany)·2026

Xanthene-Based FLPs Featuring Perchloroarylborane Substitutents: Interplay of Noncovalent Aggregation and Dihydrogen Activation.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Crack-localization in elastomeric self-healing enabled by one supermolecule-analogous molecule.

Nature communications·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us