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Protein Complex Assembly

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Updated: Oct 7, 2025

Author Spotlight: Enhancing Microinjection Needle Quality by Wet Beveling

Author Spotlight: Enhancing Microinjection Needle Quality by Wet Beveling

Published on: September 27, 2024

Protein Needles Designed to Self-Assemble through Needle Tip Engineering.

Kosuke Kikuchi¹, Tatsuya Fukuyama², Takayuki Uchihashi^3,4

¹School of Life Science and Technology, Tokyo Institute of Technology, Nagatsuta-cho 4259, Midori-ku, Yokohama, 226-8501, Japan.

Small (Weinheim an Der Bergstrasse, Germany)

|January 6, 2022

Summary

This summary is machine-generated.

Researchers engineered protein needles (PNs) with modified tips to control their self-assembly into ordered structures. This strategy enables precise construction of artificial protein assemblies by tuning protein-protein interactions.

Keywords:

2D assembly Monte Carlo simulation high-speed atomic force microscopy protein assembly protein needle

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Last Updated: Oct 7, 2025

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Polymeric Microneedle Array Fabrication by Photolithography

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Published on: November 17, 2015

Area of Science:

Biochemistry
Structural Biology
Synthetic Biology

Background:

Protein assemblies are crucial for biological systems, with artificial assemblies now constructible.
Design strategies for protein assemblies often overlook the anisotropic shapes of building blocks.

Purpose of the Study:

To develop a design strategy for controlling 2D protein assembly patterns using anisotropic protein needles (PNs).
To investigate how modifying tip-to-tip interactions of PNs influences their self-assembly behavior.

Main Methods:

Designed three types of tip-modified PNs by deleting specific protein domains (His-tag, foldon).
Observed and analyzed assembly dynamics using high-speed atomic force microscopy (HS-AFM).
Employed Monte Carlo (MC) simulations to model assembly mechanisms.

Main Results:

PNs with intact tips formed triangular lattices.
His-tag deleted PNs showed monomeric states with nematic order.
His-tag and foldon deleted PNs assembled into fibers.
MC simulations revealed cooperative interactions guiding triangular lattice formation.

Conclusions:

Tip modification of anisotropic PNs effectively controls 2D assembly patterns.
Directional interactions of anisotropic proteins offer a novel strategy for supramolecular architecture construction.