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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Oct 7, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Effective Genome Editing Using CRISPR-Cas9 Nanoflowers.

Chen Zhang1, He Ren1, Gengqi Liu1

  • 1Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, P. R. China.

Advanced Healthcare Materials
|January 6, 2022
PubMed
Summary

A novel Cas9-NF nanoflower nanoplatform delivers CRISPR-Cas9 gene editing tools efficiently into tumor cells. This nanoparticle inhibits tumor growth by downregulating oncogene expression, offering a new strategy for cancer treatment.

Keywords:
CRISPR-Cas9anti-tumor therapynanoflowerspolymeric micellestraceless release

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Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
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Area of Science:

  • Biotechnology
  • Nanomedicine
  • Molecular Biology

Background:

  • CRISPR-Cas9 gene editing holds promise for genetic disease treatment.
  • Efficient and targeted delivery of CRISPR-Cas9 is crucial for therapeutic applications.
  • Existing delivery systems face challenges in stability and controlled release.

Purpose of the Study:

  • To develop a novel nanoflower-like nanoplatform (Cas9-NF) for efficient intracellular delivery of CRISPR-Cas9.
  • To investigate the controlled release of Cas9 in response to the tumor microenvironment.
  • To evaluate the therapeutic potential of Cas9-NF in cancer treatment.

Main Methods:

  • Fabrication of mesoporous nanoflower-like nanoparticles by crosslinking Cas9 and polymeric micelles.
  • Characterization of nanoparticle morphology and stability under various conditions.
  • In vitro and in vivo evaluation of Cas9 delivery, gene editing efficiency, and anti-tumor effects in a murine tumor model.

Main Results:

  • The Cas9-NF nanoplatform demonstrated efficient intracellular delivery and controlled release of Cas9 in tumor cells.
  • Cas9-NF protected Cas9 protein from degradation under extreme pH conditions.
  • The nanoplatform effectively downregulated oncogene expression and inhibited tumor growth in vivo.
  • Tunable flower morphology was achieved by adjusting protein concentration and crosslinkers.

Conclusions:

  • The Cas9-NF nanoflower nanoplatform represents a promising system for protein delivery and gene regulation.
  • This approach offers a new pathway for cancer treatment by enabling targeted gene editing.
  • Further research is warranted to explore the full therapeutic potential of this protein-micelle crosslinked nanoflower system.