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Related Experiment Videos

The capillary thrombometer and von Willebrand factor.

R P Kadota1, H C Emslander, Y Sawada

  • 1Children's Hospital of San Diego, CA.

Thrombosis Research
|February 1, 1987
PubMed
Summary

A modified capillary thrombometer effectively measures thrombus formation rates. This study confirms von Willebrand factor

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Area of Science:

  • Hematology
  • Hemostasis Research
  • Thrombosis Studies

Background:

  • Von Willebrand factor (vWF) plays a crucial role in primary hemostasis.
  • Understanding vWF's role in thrombus formation is vital for hemostasis research.
  • Platelet storage pool disease (SPD) and von Willebrand's disease (vWD) are bleeding disorders affecting hemostasis.

Purpose of the Study:

  • To investigate the rate of thrombus formation using a modified capillary thrombometer.
  • To assess the role of von Willebrand factor in thrombus formation.
  • To correlate capillary thrombometer measurements with in vivo hemostasis.

Main Methods:

  • Utilized a modified capillary thrombometer with heparinized whole blood from normal pigs and pigs with vWD (heterozygous and homozygous) and SPD.
  • Administered cryoprecipitate to homozygous vWD pigs.
  • Applied murine monoclonal antibodies against porcine von Willebrand factor to normal pig blood.
  • Employed immunoperoxidase staining to localize von Willebrand factor in thrombi.

Main Results:

  • Significant differences in thrombosis times were observed among the pig groups.
  • Homozygous vWD pigs and SPD pigs showed prolonged thrombosis times, indicating impaired thrombus formation.
  • Cryoprecipitate infusion partially corrected thrombosis and bleeding times in vWD pigs.
  • Monoclonal antibodies against vWF prolonged thrombosis times and affected bleeding times, confirming vWF's role.
  • Plasmatic and platelet-associated vWF was detected within thrombi.

Conclusions:

  • Von Willebrand factor is essential for thrombus formation in the capillary thrombometer.
  • Capillary thrombometer measurements correlate with in vivo hemostasis, as assessed by ear bleeding time.
  • The modified capillary thrombometer is a valuable tool for studying hemostasis and thrombosis.

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