Soil application of manganese sulfate effectively reduces Cd bioavailability in Cd-contaminated soil and Cd...

Cadmium (Cd) pollution in wheat fields has caused serious food safety issues in China. Manganese (Mn)-containing materials have been widely used in paddy fields to reduce Cd accumulation in rice. However, the remediation effects of MnSO₄ in wheat fields have not been well studied and the underlying mechanisms are poorly understood. Our field experiment showed that the application of 0.1% and 0.2% MnSO₄ in soil reduced Cd concentrations significantly in wheat root, stem, leaf, and grain by 26.67-30.76%, 15.78-29.30%, 22.03-30.66%, and 30.57-50.55%, respectively, while increasing Mn concentrations significantly in these wheat tissues. MnSO₄ application significantly increased soil available Mn content by 3.78-6.19 times, the free Mn oxides and amorphous Mn oxides by 1.72-10.38 times, and Mn oxides bound Cd contents by 10.23-39.55%, resulting in a reduction of Cd availability by 30.11-40.78%. Simultaneously, MnSO₄ treatment altered the chemical forms of Cd and Mn, promoted the soluble protein concentration, and decreased the malondialdehyde (MDA) content in wheat roots. Additionally, soil application of MnSO₄ down-regulated the expression of TaNramp5, TaHMA2, and TaLCT1 in wheat roots, mediating the reduction of wheat root Cd concentration, and increased the sequestration of Cd into vacuoles by up-regulating the expression of TaHMA3. These findings add to the current knowledge of how MnSO₄ affects Cd mobilization and absorption via different mechanisms occurring both in the soil medium and at the plant level. This research indicates that soil application of MnSO₄ has great potential to remediate Cd-contaminated wheat fields.