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Updated: Oct 7, 2025
Analysis of Fatty Acid Content and Composition in Microalgae
Published on: October 1, 2013
The effect of cell disruption on the extraction of oil and protein from concentrated microalgae slurries
Ronald Halim1, Ioannis Papachristou2, George Q Chen3
1Institute for Pulsed Power and Microwave Technology (IHM), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen 76344, Germany; Institute of Process Engineering in Life Sciences, Bioprocess Engineering, Karlsruhe Institute of Technology (KIT), Karlsruhe 76131, Germany; School of Biosystems and Food Engineering, University College Dublin, Belfield, Dublin 4, Ireland; UCD Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland.
Abstract:
Novel cell-disruption combinations (autolytic incubation and hypotonic osmotic shock combined with HPH or pH12) were used to investigate the fundamental mass transfer of lipids and proteins from Nannochloropsis slurries (140 mg biomass/g slurry). Since neutral lipids exist as cytosolic globules, their mass transfer was directly dependent on disintegration of cell walls. Complete recovery was obtained with complete physical disruption. HPH combinations exerted more physical disruption and led to higher yields than pH12. In contrast, proteins exist as both cytosolic water-soluble fractions and cell-wall/membrane structural fractions and have a complex extraction behaviour. Mass transfer of cytosolic proteins was dependent on cell-wall disintegration, while that of structural proteins was governed by cell-wall disintegration and severance of protein linkage from the wall/membrane. HPH combinations exerted only physical disruption and were limited to releasing soluble proteins. pH12 combinations hydrolysed chemical linkages in addition to exerting physical disruption, releasing both soluble and structural proteins.
