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Ultrasensitive dynamic light scattering immunosensing platform for NT-proBNP detection using boronate affinity

Jiaqi Hu1,2, Lu Ding3, Jing Chen1,2

  • 1State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang, 330047, People's Republic of China.

Journal of Nanobiotechnology
|January 7, 2022
PubMed
Summary

A new dynamic light scattering (DLS) immunosensing technology detects glycoprotein N-terminal pro-brain natriuretic peptide (NT-proBNP) with high sensitivity. This method utilizes boronate affinity amplification for rapid and accurate NT-proBNP quantification.

Keywords:
AggregationBoronate affinityDynamic light scatteringImmunosensorNT-proBNP

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Glycoprotein N-terminal pro-brain natriuretic peptide (NT-proBNP) is a key biomarker for heart failure.
  • Existing immunoassay methods for NT-proBNP detection often lack sensitivity or require complex procedures.
  • There is a need for rapid, sensitive, and point-of-care diagnostic tools for NT-proBNP.

Purpose of the Study:

  • To develop a novel dynamic light scattering (DLS) immunosensing technology for NT-proBNP detection.
  • To enhance sensitivity and reduce the limit of detection (LOD) using boronate affinity amplification.
  • To create a rapid and sensitive platform for NT-proBNP quantification suitable for point-of-care applications.

Main Methods:

  • Covalent coupling of antibodies to magnetic nanoparticles (MNPs) as nanoprobes.
  • Utilizing silica nanoparticles modified with phenylboronic acid (SiO2@PBA) as crosslinking agents.
  • Employing boronate affinity recognition between boronic acid ligands and cis-diols on NT-proBNP to induce nanoparticle aggregation.
  • Dynamic light scattering (DLS) for signal transduction and quantification of NT-proBNP.

Main Results:

  • Achieved an ultrahigh sensitivity with a limit of detection (LOD) of 7.4 fg mL⁻¹.
  • Demonstrated a fast response time of less than 20 minutes.
  • Required minimal sample consumption (1 μL).
  • The developed DLS immunosensor exhibited excellent selectivity, accuracy, precision, reproducibility, and practicability.

Conclusions:

  • The developed boronate affinity amplified-DLS immunosensor offers superior performance compared to traditional immunoassays.
  • This technology provides a promising approach for the rapid and sensitive detection of NT-proBNP.
  • The platform is suitable for field or point-of-care testing of cis-diol-containing molecules.