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Pediatric Reference Intervals for Point-of-Care Random Glucose in Healthy Children and Adolescents

Siobhan Wilson1,2, Hannah Earle2, Mary Kathryn Bohn1,2

  • 1CALIPER Program, Department of Pediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, ON, Canada.

The Journal of Applied Laboratory Medicine
|January 7, 2022
PubMed

Insights

This study established pediatric reference intervals for random glucose testing using point-of-care instruments. Results show excellent agreement between devices, aiding accurate clinical decisions in children.

Area of Science:

  • Clinical Chemistry
  • Pediatric Diagnostics
  • Point-of-Care Testing

Background:

  • Accurate glucose reference intervals (RIs) are crucial for clinical decision-making in diagnosing and monitoring various conditions.
  • Point-of-care (POC) glucose testing is widely used but requires validated RIs for pediatric populations.
  • The Canadian Laboratory Initiative on Pediatric Reference (CALIPER) cohort provided a basis for establishing these RIs.

Purpose of the Study:

  • To establish pediatric reference intervals for random whole blood glucose using two modern POC instruments.
  • To analytically compare the performance of these POC instruments against a laboratory-based analyzer.
  • To ensure accurate glucose measurements for pediatric patient care.

Main Methods:

  • Recruited approximately 400 healthy children and adolescents (birth to 18 years).
  • Collected random venous blood samples and analyzed them on Nova Biomedical StatStrip, Radiometer ABL90 FLEX Plus (POC), and Ortho Vitros 5600 (laboratory).
  • Established RIs and performed method comparisons following CLSI guidelines.

Main Results:

  • Age-specific RIs were necessary, particularly for infants (0-<1 month, 1-<6 months, 6 months-<19 years).
  • Excellent concordance (Pearson r > 0.90) was found between the two POC platforms.
  • Good comparability (Pearson r > 0.80) was observed between POC and laboratory-based platforms.

Conclusions:

  • Established comprehensive pediatric RIs for random glucose (whole blood) on modern POC systems for the first time within the CALIPER cohort.
  • Demonstrated excellent concordance between POC systems and good comparability with laboratory analyzers.
  • These findings will support more accurate clinical decision-making in pediatric healthcare.
Abstract

Keywords:
CALIPERPediatriccritical careglucosepoint of carereference interval

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