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Isolation and Maintenance of Tumor-Infiltrating Lymphocytes for Translational and Clinical Applications: Established

Orenthial J Fulbright1, Marie-Andrée Forget1, Cara Haymaker1

  • 1Department of Melanoma Medical Oncology, The University of Texas MD Anderson Cancer Center (MDACC), Houston, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 7, 2022
PubMed

Adoptive cell transfer (ACT) of in vitro expanded tumor-infiltrating lymphocytes (TIL) for the treatment of patients with advanced stages of metastatic melanoma remains one of the most beneficial therapies eliciting long-lasting responses. Methods and protocols used to expand TIL have evolved over time, utilizing different culture devices and other tools, to streamline and maximize the end product in both numbers and quality. Summarized in this chapter are the latest protocols used in the TIL program at MDACC.

Keywords:
Adoptive cell therapy (ACT )Cell cultureCytotoxic T-lymphocytesImmunotherapyRapid expansion protocol (REP )Tumor-infiltrating lymphocytes (TIL)

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