Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

6.9K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.9K
Proteomics01:33

Proteomics

8.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct quantification of waterborne viruses via high-temperature and high-pressure treatment: a simplified nucleic acid extraction-free approach.

Scientific reports·2026
Same author

Pre-infusion plasma proteomics identifies an endothelial-immune priming signature predictive of severe cytokine release syndrome and neurotoxicity following CAR T-cell therapy in relapsed/refractory lymphoma.

medRxiv : the preprint server for health sciences·2026
Same author

Simultaneous detection of protozoa, bacteria, and viruses from environmental water through membrane-adsorption followed by direct nucleic acid extraction.

Water research·2026
Same author

A pilot study of a digital weight loss program for people with obesity in four diverse cohorts.

Journal of behavioral medicine·2026
Same author

Blood-Based Immunoglobulin-Bound Neoantigen Signatures Associated with MEN Type 1-Related Duodenopancreatic Neuroendocrine Tumor Progression.

Journal of the American College of Surgeons·2026
Same author

A full-scale Co-located comparison of virus removal by membrane bioreactor and conventional activated sludge systems.

Water research·2026

Related Experiment Video

Updated: Oct 7, 2025

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
08:32

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Published on: June 7, 2018

9.8K

Proteomic Profiling of the Tumor Microenvironment.

Michela Capello1, Hiroyuki Katayama1, Samir M Hanash2

  • 1Departments of Clinical Cancer Prevention, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 7, 2022
PubMed
Summary

This study presents a novel proteomic method to analyze cell populations within the tumor microenvironment. This approach aids in understanding tumor progression and identifying new therapeutic targets.

Keywords:
Chromatographic fractionationMass spectrometryProteomicsSILACTumor microenvironment

More Related Videos

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

1.3K
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
08:08

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

16.5K

Related Experiment Videos

Last Updated: Oct 7, 2025

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
08:32

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Published on: June 7, 2018

9.8K
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

1.3K
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
08:08

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

16.5K

Area of Science:

  • Oncology
  • Proteomics
  • Cell Biology

Background:

  • The tumor microenvironment (TME) is a complex milieu supporting tumor growth.
  • Understanding TME composition and cell interactions is crucial for cancer research.
  • Current proteomic methods face challenges in dissecting TME heterogeneity.

Purpose of the Study:

  • To develop and validate a method for profiling individual cell populations within the TME.
  • To investigate protein expression and localization changes in TME cells.
  • To identify novel pathways and diagnostic markers for cancer progression.

Main Methods:

  • Isolation of individual cell compartments from tumor tissue.
  • Chromatographic fractionation of intact proteins.
  • Enzymatic digestion and mass-spectrometry analysis of fractions.
  • Proteomic profiling of specific TME cell populations.

Main Results:

  • The method successfully profiled proteins from distinct TME cell compartments.
  • Differential protein expression and localization were identified.
  • The study revealed communication mechanisms within the TME.

Conclusions:

  • This proteomic approach enables detailed characterization of the TME.
  • It facilitates the identification of clinically targetable pathways.
  • The method supports the development of new diagnostic and therapeutic strategies in oncology.