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Updated: Oct 7, 2025

An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines
Published on: October 11, 2022
Rickettsiae in fleas infesting domestic pets of eastern Himalayan terrains of India
Siraj A Khan1, Jahnabi Saikia1, Trishna Bora1
1Department of Medical Entomology, Arbovirology and Rickettsial diseases, Indian Council of Medical Research-Regional Medical Research Centre, Northeast Region, Post Box 105, Dibrugarh 786010, Assam, India.
Background:
Flea-borne rickettsioses have been limitedly explored in the Indian sub-Himalayan belt, including the North Eastern Region (NER) of India. This study investigates the presence of rickettsiae hosts and their probable pathogens in the disease-endemic hilly state of the NER of India.
Methods:
Entomological surveys were carried out in disease-reporting localities in a hilly state in India. Fleas collected from domesticated animals were processed for detection of a Rickettsia-specific 17-kDa gene.
Results:
Sequence analysis revealed Rickettsia felis in six flea pools (40%), Candidatus Rickettsia senegalensis in two pools (13.3%) and Rickettsia asembonensis in one pool (6.6%).
Conclusions:
Our findings suggest Ctenocephalides felis, Ctenocephalides canis and Pulex irritans as potential carriers of R. felis and R. felis-like organisms in India.
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