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Updated: Oct 7, 2025
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Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
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Transcription factor c-Myb: novel prognostic factor in osteosarcoma
Kamila Říhová1, Monika Dúcka1,2, Iva Staniczková Zambo2,3
1Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Clinical & Experimental Metastasis
|January 7, 2022
Summary
High c-Myb expression correlates with poor prognosis in osteosarcoma patients. This transcription factor drives tumor growth, migration, and metastasis, suggesting it as a therapeutic target for osteosarcoma progression.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- The transcription factor c-Myb (MYB) is an oncoprotein implicated in various cancers.
- While c-Myb is expressed in osteosarcoma cell lines, its role in osteosarcoma progression is not well understood.
Purpose of the Study:
- To investigate the prognostic significance of c-Myb in high-grade osteosarcoma.
- To elucidate the functional role of c-Myb in osteosarcoma cell growth, chemosensitivity, migration, and metastasis.
Main Methods:
- Retrospective analysis of c-Myb expression in archival osteosarcoma tissues.
- In vitro depletion of MYB in osteosarcoma cell lines (SAOS-2 LM5, 143B).
- In vivo assessment of metastatic activity in immunodeficient mice.
- Transcriptomic analysis to identify c-Myb-regulated pathways.
Main Results:
- High c-Myb expression was significantly associated with poor overall survival and metastatic progression in young osteosarcoma patients.
- MYB depletion reduced osteosarcoma cell growth, colony formation, migration, and chemoresistance in vitro.
- MYB knock-out decreased the metastatic potential of osteosarcoma cell lines in vivo.
- Transcriptomic analysis identified c-Myb-driven programs regulating cell growth, stress response, adhesion, differentiation, and the Wnt signaling pathway.
Conclusions:
- c-Myb is a negative prognostic factor in osteosarcoma.
- c-Myb plays a critical role in regulating osteosarcoma cell growth, chemosensitivity, migration, and metastatic activity.
- Targeting c-Myb may offer a therapeutic strategy for osteosarcoma.
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