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Updated: Oct 7, 2025

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Mixotrophic cultivation of Monoraphidium sp. In dairy wastewater using Flat-Panel photobioreactor and photosynthetic
Sri Divya Kuravi1, S Venkata Mohan1
1Bioengineering and Environmental Sciences Lab, Department of Energy and Environmental Engineering (DEEE), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Abstract:
Monoraphidium sp. SVMIICT6 was isolated and mixotrophically cultivated in a flat-panel photobioreactor (8 days) to treat synthetic dairy wastewater. COD, nitrates, and phosphates removal efficiencies were 75%, 85%, and 60% respectively. The nutrient removal supported the growth of microalgae in terms of biomass productivity (50 mg L-1d-1), and accumulation of carbohydrate (228.8 mg g-1), protein (88.8 mg g-1), and lipid content (25%). Elemental analysis of microalgal biomass revealed carbon (50.6%) as a major fraction. Quantum yield and electron transport rate (ETR) from PSII to PSI increased with time correlating well with chlorophyll pigments (89.53 mg g-1). The lipid profile resulted in a major fraction of Heptadecanoic acid (C17:0; 51.5%), followed by Myristoleic acid (C14:1; 24.3%) with potent nutraceutical properties. The isolated strain showed efficient treatment of dairy wastewater yielding biomass for diverse applications.

