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Published on: July 17, 2014
A benzothiazole-based fluorescent and colorimetric probe for the detection of ClO- and its application to zebrafish
Boeon Suh1, Hyeongjin Kim1, Soogyeong Jang2
1Department of Fine Chem., SNUT (Seoul National Univ. of Sci. and Tech.), Seoul 01811, South Korea.
Abstract:
A benzothiazole-based fluorescent and colorimetric chemosensor BZD ((E)-2-(benzo[d]thiazol-2-yl)-5-((4-(diethylamino)-2-hydroxybenzylidene)amino)phenol) was applied for detecting ClO-. BZD showed fluorescence quenching and color variation for ClO- via oxidative reaction between ClO- and the imine bond. It could effectively detect ClO- over various competitive analytes. Detection limit for ClO- was calculated to be 1.74 μM by fluorescent method and 16.44 μM by colorimetric one, respectively. Additionally, BZD could be utilized for sensing ClO- in zebrafish, real water sample and paper strip. The photophysical characteristics and sensing mechanism of BZD to ClO- were studied by fluorescent and UV-visible spectroscopy, NMR titration, and ESI-mass spectrometry.
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