Ascorbic Acid-Loaded Apoferritin-Assisted Carbon Dot-MnO2 Nanocomposites for the Selective and Sensitive Detection of
Qingqing Tan1, Ruirui Zhang2, Weisu Kong1
1College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, People's Republic of China.
Abstract:
A novel "turn-on" fluorescent trypsin detection platform dependant on carbon dot-MnO2 (CD-MnO2) nanocomposites and ascorbic acid-loaded apoferritin (APOAA) was fabricated. The detection mechanism relied on trypsin-catalyzed enzymolysis of APOAA, which released ascorbic acid (AA) as a reducing agent to disintegrate the MnO2 nanosheets, causing the recovery of the fluorescence of CDs. An excellent performance and high sensitivity of trypsin determination were observed with a detection limit (LOD) of 0.3411 ng/mL. This work provides us with a unique strategy for trypsin detection in human serum samples, which reveals the potential applications in clinical detection.
