Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A vascularized modular 3D <i>in vitro</i> liver model to study bacterial infection and the role of associated host protein markers.

Journal of materials chemistry. B·2026
Same author

SERS active silk silver films as a platform for osteogenic biomarker detection.

Journal of materials chemistry. B·2026
Same author

Smart Silica Nanocomposites Activating Plant Defenses via Enhanced Biomolecule Deposition on Superhydrophobic Monocot Surfaces.

Journal of agricultural and food chemistry·2025
Same author

A Rapid Electrochemical Immunosensor Platform for the Sepsis-Associated Host and Pathogen Marker Dual Detection.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Electrochemical Biosensors for Sepsis Detection in Liver Cirrhosis: Advances and Insights.

ACS applied bio materials·2025
Same author

Bioinspired Silica Nanoparticles for Enhanced Adhesion and Retention of Biomolecules: A Sustainable and Green Strategy for Disease Management.

ACS applied bio materials·2025

Related Experiment Video

Updated: Oct 7, 2025

Silk Film Culture System for in vitro Analysis and Biomaterial Design
11:19

Silk Film Culture System for in vitro Analysis and Biomaterial Design

Published on: April 24, 2012

19.6K

Self-Patterning Silk Films for Cellular Coculture.

Vartika Dhyani1, Neetu Singh1,2

  • 1Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, Delhi, New Delhi 110016, India.

ACS Applied Bio Materials
|January 8, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel method using silk nanogels to create patterned surfaces for tissue engineering. This technique supports stem cell adhesion and differentiation, offering a simpler alternative to traditional methods.

Keywords:
Bombyx moricoculturepatterningsilk filmsstem cells

More Related Videos

Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization
08:02

Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization

Published on: July 3, 2018

10.8K
Author Spotlight: Enhancing In Vitro Cell Culture Models with Recombinant Functionalized Spider Silk Membranes
06:17

Author Spotlight: Enhancing In Vitro Cell Culture Models with Recombinant Functionalized Spider Silk Membranes

Published on: November 1, 2024

1.3K

Related Experiment Videos

Last Updated: Oct 7, 2025

Silk Film Culture System for in vitro Analysis and Biomaterial Design
11:19

Silk Film Culture System for in vitro Analysis and Biomaterial Design

Published on: April 24, 2012

19.6K
Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization
08:02

Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization

Published on: July 3, 2018

10.8K
Author Spotlight: Enhancing In Vitro Cell Culture Models with Recombinant Functionalized Spider Silk Membranes
06:17

Author Spotlight: Enhancing In Vitro Cell Culture Models with Recombinant Functionalized Spider Silk Membranes

Published on: November 1, 2024

1.3K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Silk from Bombyx mori is a natural polymer with significant potential in biomedical applications.
  • Tissue engineering demands precise control over cellular adhesion, often achieved through complex lithographic methods.
  • Existing techniques for patterning surfaces can be time-consuming and involve multiple processing steps.

Purpose of the Study:

  • To develop a direct, efficient method for creating biocompatible patterned surfaces for tissue engineering.
  • To investigate the use of silk-based nanogels for controlled cellular growth and interaction.
  • To provide a platform favoring stem cell adhesion, differentiation, and co-culture.

Main Methods:

  • Utilized silk derived from Bombyx mori to form nanogels.
  • Employed photoactive groups for facile cross-linking of nanogels into patterned structures.
  • Developed a direct patterning approach for creating biocompatible surfaces.

Main Results:

  • Successfully formed stable, biocompatible patterned systems using cross-linked silk nanogels.
  • Demonstrated the ability of the patterned surface to support stem cell adhesion and differentiation.
  • Showcased the potential for spatially controlled co-culture with an additional cell type.

Conclusions:

  • The developed nanogel cross-linking technique offers a streamlined approach to creating patterned surfaces for tissue engineering.
  • This method facilitates controlled stem cell behavior and enables complex cellular arrangements.
  • The approach holds promise for advancing regenerative medicine and tissue fabrication.