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Related Experiment Video

Updated: Oct 7, 2025

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
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A Simple Fluorescent Aptasensing Platform Based on Graphene Oxide for Dopamine Determination.

Ahlem Teniou1, Amina Rhouati2, Gaëlle Catanante3

  • 1Bioengineering Laboratory, Higher National School of Biotechnology, Constantine, Algeria.

Applied Biochemistry and Biotechnology
|January 8, 2022
PubMed
Summary

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This study presents a novel fluorescent aptasensor for detecting dopamine (DA). The graphene oxide-based sensor offers a rapid, sensitive, and selective method for dopamine determination, crucial for disease diagnosis.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Dopamine (DA) is a vital neurotransmitter implicated in numerous physiological processes.
  • Accurate detection of DA is critical for diagnosing diseases linked to its abnormal levels.
  • Existing detection methods require improvement in speed, simplicity, and sensitivity.

Purpose of the Study:

  • To develop a highly sensitive and selective fluorescent aptasensor for rapid dopamine detection.
  • To utilize graphene oxide (GO) as a quencher in a fluorescence resonance energy transfer (FRET) system.
  • To establish a reliable method for dopamine quantification in biological samples.

Main Methods:

  • A fluorescent aptasensor was designed using graphene oxide (GO) as a quencher and a carboxy fluorescein (FAM)-labeled aptamer as the signal reporter.
Keywords:
AptamerDopamineFluorescence quenchingFluorescence resonance energy transferGraphene oxide

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  • The sensor operates on the principle of FRET, where aptamer adsorption on GO quenches FAM fluorescence.
  • Dopamine binding induces aptamer conformational change, releasing it from GO and restoring fluorescence.

    • Main Results:

    • The aptasensor demonstrated a linear response to dopamine in the range of 3-1680 nM.
    • A low limit of detection (LOD) of 0.031 nM and a limit of quantification (LOQ) of 0.1 nM were achieved.
    • The assay showed minimal interference from other substances and proved applicable to human serum samples.

    Conclusions:

    • The developed GO-based fluorescent aptasensor provides a promising tool for sensitive and selective dopamine detection.
    • This method offers a rapid, simple, and accurate approach for potential clinical diagnostics.
    • The aptasensor's applicability in human serum highlights its potential for real-world applications.