Mechanical properties of brain tissue based on microstructure

Research on the mechanical properties of brain tissue has gradually deepened recently. Two indentation protocols were used here to characterize the mechanical properties of cortical tissues. Further, histological staining was used to explore the correlation between the mechanical properties and microstructure on the basis of the density of cell nuclei and proteoglycan content. No significant difference was observed in transient contact stiffness between the cerebral cortex and cerebellar cortex at the depth interval of 0-600 μm under the cortical surface; however, the average shear modulus of the cerebral cortex was higher than that of the cerebellar cortex. The cerebral cortex responded more quickly to the change in load and released stress more thoroughly than the cerebellar cortex. In addition, the density of cell nuclei was related to both the transient contact stiffness and second time constant of cortical tissues. Proteoglycan content had a more significant impact on the shear modulus, second time constant, and stress relaxation rate of cortical tissues. Exploring mechanical properties thoroughly will provide more detailed mechanical information for future brain chip implantation. Alternatively, linking the mechanical properties of cortical tissues to the microstructure can provide basic data for the design and manufacture of substitute materials for brain tissue.