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Deduction of CDC42EP3 suppress development and progression of osteosarcoma

Peng Xu1, Xiaoxi Li2, Chao Tang3

  • 1Department of Sports Medicine, The First Hospital of Jilin University, Jilin University, 71 Xinmin Street, Chaoyang District, Changchun City, Jilin Province, China.

Experimental Cell Research
|January 9, 2022
PubMed
Abstract

Insights

CDC42 effector protein 3 (CDC42EP3) promotes osteosarcoma progression by increasing proliferation and migration. Downregulating CDC42EP3 inhibits tumor growth, suggesting it as a potential therapeutic target for osteosarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Osteosarcoma is a highly fatal cancer in children and adolescents.
  • CDC42 effector protein 3 (CDC42EP3) is implicated in human cancer progression.
  • The role of CDC42EP3 in osteosarcoma remains largely uncharacterized.

Purpose of the Study:

  • To investigate the biological function of CDC42EP3 in osteosarcoma.
  • To elucidate the preliminary molecular mechanisms of CDC42EP3 in osteosarcoma.
  • To evaluate CDC42EP3 as a potential therapeutic target for osteosarcoma.

Main Methods:

  • Immunohistochemical (IHC) staining to analyze CDC42EP3 expression in osteosarcoma tissues.
  • In vitro and in vivo loss/gain-of-function assays to assess the biological effects of CDC42EP3.
  • Western blotting to examine the expression of epithelial-mesenchymal transition (EMT) markers and the PI3K/AKT/mTOR pathway.

Main Results:

  • CDC42EP3 expression is elevated in osteosarcoma tissues and correlates with advanced stage and grade.
  • Downregulation of CDC42EP3 inhibits osteosarcoma cell proliferation, migration, and induces apoptosis.
  • CDC42EP3 knockdown reduces mesenchymal markers, increases epithelial markers, and downregulates the PI3K/AKT/mTOR pathway.

Conclusions:

  • CDC42EP3 plays a significant role in promoting osteosarcoma progression.
  • CDC42EP3 inhibition suppresses tumor growth and EMT.
  • CDC42EP3 represents a promising molecular target for osteosarcoma therapy.
Keywords:
ApoptosisCDC42EP3MigrationOsteosarcomaProliferation

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