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Updated: Oct 7, 2025

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
Published on: April 17, 2012
A novel method for multiplex protein biomarker analysis of human serum using quantitative MALDI mass spectrometry
Aleksandr S Taraskin1, Konstantin K Semenov2, Alexey A Lozhkov1
1Smorodintsev Research Institute of Influenza, div. Russian Ministry of Health, 15/17 Ulitsa Professora Popova, St. Petersburg 197376, Russia; Peter the Great St. Petersburg Polytechnic University, 29 Ulitsa Polytechnicheskaya, St. Petersburg 194064, Russia.
Abstract:
In this work, we have extended our previously proposed approach for determining protein concentrations in human serum (using MALDI-TOF mass spectrometry) to include simultaneous analysis of several proteins associated with acute inflammation (alpha-2-macroglobulin, fetuin-A, serum amyloid A1). This technique can be used to diagnose systemic inflammation and provides results in 4-5 h. The developed approach was verified using standard immunological methods (ELISA). Samples from 87 individuals, in specific groups, were used for testing and validation: control; inflammatory soft tissue disease accompanied by sepsis; influenza A infection; or COVID-19. The feasibility of differentiating patient groups with the aforementioned conditions was analyzed using a combination of the inflammatory markers described. For fetuin-A and serum amyloid A1, diagnostically significant concentration ranges were established.
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