Permeability of endoneurial capillaries to K, Na and Cl and its relation to peripheral nerve excitability

The permeability coefficient-surface area products (PA) of frog sciatic nerve endoneurial capillaries to K, Na and Cl were measured with an in situ perfusion technique and found to be 40.3, 24.6 and 32.8 X 10(-5) ml . g-1 . s-1, respectively. PAs to [14C]sucrose and 42K, when measured simultaneously, and their ratio were independent of perfusate K concentration (0.1-10.0 mM). Simultaneous measurements with 36Cl and 42K indicated that the Cl/K permeability ratio was significantly smaller than the mobility ratio of these two ions in free solution. On the other hand, comparable experiments with 22Na and 42K revealed that the K/Na permeability ratio was not significantly different from its respective mobility ratio. Thus, these results provide no evidence of facilitated transport of K by endoneurial capillaries, and suggest that K, Na and Cl traverse the endoneurial capillary wall by a paracellular route which is weakly selective for cations. The minimum extracellular K concentration (Ke) capable of producing a depolarization conduction block in frog sciatic nerve was between 12.5 and 15.0 mM. When the vasculature of this nerve was perfused with a hyperkalaemic (20.0 mM) Ringer solution, a conduction block developed in 7.9 min. Comparison of this time with the theoretically predicted rate of change of endoneurial Ke (induced by a comparable change of intravascular K concentration) indicated that an increase of endoneurial Ke is transmitted directly to the paranodal spaces of nerve fibres so as to immediately influence axonal excitability.(ABSTRACT TRUNCATED AT 250 WORDS)