Captopril inhibits matrix metalloproteinase activity and improves dentin bonding durability

Area of Science:

• Dental Materials Science
• Biomaterials Engineering
• Biochemistry

Background:

• Dental bonding durability is often compromised by the activity of matrix metalloproteinases (MMPs) in dentin.
• Inhibiting MMPs is crucial for improving the long-term success of dental adhesives.

Purpose of the Study:

• To investigate the inhibitory effects of captopril on dentin MMPs.
• To evaluate captopril's efficacy as a primer for enhancing dentin bonding durability.

Main Methods:

• Human third molars were treated with primers including captopril, chlorhexidine digluconate (CHD), or water.
• MMP inhibitory effects were assessed using hydroxyproline and gelatinase activity tests, and in situ zymography.
• Dentin bonding durability was evaluated through microtensile bond strength and nanoleakage tests after 1, 12, and 24 months of aging.

Main Results:

• Captopril demonstrated superior inhibition of MMPs compared to 2% CHD.
• The 0.2% captopril aqueous solution (0.2% CapW) significantly reduced MMP activity.
• Microtensile bond strength was maintained longer in the captopril group, with significantly fewer interface defects detected by nanoleakage.

Conclusions:

• Captopril effectively inhibits dentin MMP activity.
• Captopril significantly improves dentin bonding durability and reduces interface defects.
• Captopril shows promise as an effective primer for long-lasting dental restorations.