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Related Experiment Videos

B7, a B-cell-restricted antigen that identifies preactivated B cells.

A S Freedman1, G Freeman, J C Horowitz

  • 1Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115.

Journal of Immunology (Baltimore, Md. : 1950)
|November 15, 1987
PubMed
Summary

Researchers identified a new B cell surface protein, B7 antigen, appearing within 24 hours of stimulation. This B7 antigen marks a subset of B cells with an accelerated proliferation response, suggesting they are primed in vivo.

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Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Activated B cells express unique surface proteins not found on resting cells.
  • Several B lineage-restricted activation antigens have been identified, appearing at different times post-activation.

Purpose of the Study:

  • To describe a novel B lineage-restricted activation antigen, designated B7.
  • To characterize its expression pattern, biochemical properties, and functional significance.

Main Methods:

  • In vitro stimulation of B cells with antigen or mitogens.
  • Flow cytometry for antigen detection.
  • Biochemical analysis to determine protein characteristics.
  • Cell proliferation assays.

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Main Results:

  • A new B lineage-restricted antigen (B7) was identified, appearing within 24 hours of in vitro stimulation.
  • B7 expression was induced by anti-immunoglobulin or Epstein-Barr virus and found on a minor B cell subpopulation.
  • B7 was not detected on T cells or monocytes but was present on some B cell lines and neoplasms.
  • Biochemical analysis revealed B7 as a 60 kDa single-chain protein, distinct from other B cell activation antigens.
  • B7+ B cells exhibited an earlier peak proliferation response to anti-Ig stimulation compared to B7- cells.

Conclusions:

  • B7 antigen identifies a subpopulation of B cells that are preactivated or primed in vivo.
  • These B7+ cells demonstrate an accelerated response to activation stimuli.
  • B7 serves as a unique marker for a specific subset of activated B cells.