Establishment and Clinical Application of a Method for Detecting T Lymphocyte Subsets by Cellular Immunochip...

Area of Science:

• Immunology
• Clinical Diagnostics
• Biotechnology

Background:

• Peripheral blood T lymphocyte immune phenotyping is crucial for assessing immune status.
• Existing methods like flow cytometry can be time-consuming and complex.
• A need exists for efficient and accurate T lymphocyte subset detection in clinical settings.

Purpose of the Study:

• To establish and validate a cellular immune chip technology for detecting peripheral blood T lymphocyte subsets (CD3+, CD4+, CD8+, CD4/CD8 ratio).
• To analyze the immune status and clinical diagnostic value of this method in diverse populations within the Qingyuan area.
• To compare the performance of cellular immune chip technology against flow cytometry.

Main Methods:

• Cellular immune chip technology was employed to quantify T lymphocyte subsets (CD3+, CD4+, CD8+, CD4/CD8).
• Accuracy and precision were verified by comparing results with flow cytometry.
• Large-scale detection was conducted on 8389 cases to analyze immune function differences across various groups.

Main Results:

• The cellular immune chip method demonstrated comparable accuracy and precision to flow cytometry, offering speed and simplicity for clinical application.
• Statistical differences in T lymphocyte subset counts were observed among individuals undergoing physical examinations, those with inflammation, and cancer patients.
• Variations in T cell counts and CD4/CD8 ratios were noted between different cancer types and diseases.

Conclusions:

• Cellular immune chip technology provides a practical, accurate, and rapid method for detecting T lymphocyte subsets.
• This technology is suitable for immune function monitoring and prognosis evaluation in patients with various diseases.
• The method holds significant potential for widespread clinical application and popularization.