Indication-based protocols with different solutions for mechanical stromal-cell transfer

Area of Science:

• Regenerative Medicine
• Plastic Surgery
• Cell Biology

Background:

• Regenerative medicine is a rapidly advancing field within plastic surgery.
• Adipose tissue is a primary source of stromal cells.
• Current mechanical isolation methods lack standardized protocols.

Purpose of the Study:

• To develop standardized indication-based protocols (IPs) for adipose tissue processing.
• To introduce mechanical stromal-cell transfer (MEST) using ultra-sharp blades.
• To evaluate the impact of diluents (saline, Ringer, 5% Dextrose) on cell yield and viability.

Main Methods:

• Developed four distinct IPs for achieving desired physical states (liquid, gel, solid) and volumes.
• Employed ultra-sharp blades to minimize tissue trauma during mechanical isolation.
• Utilized pre-dilution of adipose tissue with saline, Ringer's, or 5% Dextrose solutions in specific IPs.

Main Results:

• MEST achieved a mean stromal cell viability of 92% from 100 mL of adipose tissue.
• Stromal cell counts ranged from 26.80–91.90 × 10^6 cells.
• The four IPs successfully yielded stromal cells in desired forms and quantities.

Conclusions:

• Mechanical isolation using sharp blades preserves stromal cell viability and increases cell counts.
• Pre-dilution of adipose tissue with solutions like saline, Ringer's, or 5% Dextrose further enhances stromal cell yield.
• These advancements provide clinicians with adaptable stromal cell solutions for diverse regenerative applications.