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Related Experiment Video

Updated: Oct 7, 2025

Retinal Explant of the Adult Mouse Retina as an Ex Vivo Model for Studying Retinal Neurovascular Diseases
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CCRL2 Modulates Physiological and Pathological Angiogenesis During Retinal Development.

Cyrine Ben Dhaou1,2, Annalisa Del Prete3, Silvano Sozzani4,5

  • 1WELBIO and I.R.I.B.H.M., Université Libre de Bruxelles, Brussels, Belgium.

Frontiers in Cell and Developmental Biology
|January 10, 2022
PubMed
Summary
This summary is machine-generated.

The atypical receptor CCRL2 regulates chemerin levels and bioactivity, impacting retinal vascular development. Mice lacking CCRL2 show reduced blood vessel density, highlighting CCRL2

Keywords:
CCRL2CMKLR1ChemR23G protein-coupled receptors (GPCRs)chemerinoxygen-induced retinopathyretinal angiogenesis

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Area of Science:

  • Vascular Biology
  • Endocrinology
  • Immunology

Background:

  • Chemerin is a protein regulating inflammation, metabolism, and tumorigenesis.
  • Chemerin interacts with CMKLR1, GPR1, and CCRL2 receptors.
  • CCRL2 is an atypical receptor that presents chemerin to CMKLR1-expressing cells and is upregulated by inflammation.

Purpose of the Study:

  • To investigate the role of the atypical receptor CCRL2 in angiogenesis.
  • To determine the impact of CCRL2 deficiency on retinal vascular development and function.

Main Methods:

  • Analysis of retinal vascularization in CCRL2 knockout mice.
  • Assessment of vascular sprouting, pruning, and endothelial cell apoptosis.
  • Evaluation of pathological angiogenesis in a mouse model of oxygen-induced retinopathy.

Main Results:

  • Mice lacking CCRL2 exhibit reduced retinal vessel density, persisting into adulthood.
  • Vascular pruning and endothelial cell apoptosis were increased in CCRL2-deficient mice.
  • Pathological angiogenesis was reduced in CCRL2 knockout mice, mimicking chemerin overexpression phenotypes.

Conclusions:

  • CCRL2 plays a critical role in regulating retinal vascular development.
  • CCRL2 influences chemerin distribution and concentration, thereby modulating its bioactivity.
  • CCRL2 deficiency impacts angiogenesis in both physiological and pathological conditions.