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Methionine Restriction Improves Gut Barrier Function by Reshaping Diurnal Rhythms of Inflammation-Related Microbes in

Bo Ren1,2, Luanfeng Wang1, Aiziguli Mulati1

  • 1Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, China.

Frontiers in Nutrition
|January 10, 2022
PubMed

Insights

Dietary methionine restriction (MR) improves gut barrier function in aging mice by reducing inflammation and restoring beneficial gut microbes. This nutritional intervention may combat age-related gut dysfunction.

Area of Science:

  • Gerontology
  • Gastroenterology
  • Microbiology

Background:

  • Age-related gut barrier dysfunction and dysbiosis are key factors in aging.
  • Dietary methionine restriction (MR) is known to extend lifespan and reduce inflammation, but its effects on gut barrier health are unclear.

Purpose of the Study:

  • To investigate the impact of MR on age-related gut barrier dysfunction, inflammation, and microbiome alterations in mice.

Main Methods:

  • A 3-month methionine restriction diet was administered to aged mice.
  • Serum inflammatory factors, gut permeability, tight junction protein mRNA levels, and lipopolysaccharide concentrations were measured.
  • 16S rRNA sequencing analyzed gut microbiome diurnal rhythmicity and microbial composition.

Main Results:

  • MR reduced serum inflammatory factors and gut permeability in aged mice.
  • MR increased tight junction protein mRNA levels (occludin, claudin-1, zona occludens-1) and decreased serum lipopolysaccharide.
  • MR partially restored disrupted gut microbiome diurnal fluctuations, enhancing beneficial microbes and dampening inflammation-related bacteria.

Conclusions:

  • Methionine restriction alleviates age-related gut barrier dysfunction by reducing inflammation and restoring microbiome rhythmicity.
  • MR represents a potential nutritional intervention strategy against age-related gut barrier decline.
Keywords:
aginggut barrierinflammationmethionine restrictionmicrobiome diurnal rhythmicity

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