A Simple Method for in situ Quantification of Cells on Carriers
Osnat Rosen1, Avital Jayson1, Niva Natan1
1Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona 7410001, Israel.
Bio-Protocol
|January 10, 2022
Summary
A new Alamar blue dye method enables simple, rapid, and in situ quantification of cell number, viability, and proliferation on various cell carriers without specialized equipment.
Area of Science:
- Biotechnology
- Cell Biology
- Bioprocess Engineering
Background:
- Cell carriers are crucial for high-density cell cultures in bioproduction.
- Quantifying cell number, viability, and proliferation is essential for process monitoring.
- Traditional assays struggle with diverse carrier structures and require specialized equipment.
Purpose of the Study:
- To develop a universal method for quantifying cells on various carriers.
- To provide a simple, rapid, and equipment-free assay for cell-based production.
- To enable accurate monitoring of cell growth and activity in situ.
Main Methods:
- Utilized Alamar blue dye, a common indicator of cell metabolic activity.
- Developed an in situ quantification technique directly on cell carriers.
- Avoided cell separation from carriers and the need for specialized instrumentation.
Main Results:
- The Alamar blue method accurately quantifies cell numbers on carriers.
- The assay provides insights into cell viability and proliferation.
- The technique is broadly applicable across different cell carrier types.
Conclusions:
- The Alamar blue dye assay offers a versatile and efficient solution for cell quantification on carriers.
- This method simplifies process control in cell-based production.
- It is adaptable for various cell-based platforms and unique growth environments.
Keywords:
Alamar blueCarriersCellsFluorescenceHigh-throughput quantificationPlate readerQuantification
