Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stress granule phase separation in stress-responsive cytosolic extract-in-oil droplets.

Nature communications·2026
Same author

Metal-Coordinated His-Tag Functionalization of Polymeric Nanogels for Therapeutic Applications.

ACS applied nano materials·2026
Same author

Deterministic droplet-based co-encapsulation of single cells through inertial and hydrodynamic focusing.

The Analyst·2026
Same author

Probing hydrogel microstructure and porosity with fluorinated liquid NMR nanocapsules.

Soft matter·2025
Same author

PiP-Plex: A Particle-in-Particle System for Multiplexed Quantification of Proteins Secreted by Single Cells.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Activation of NF-κB Signaling by Optogenetic Clustering of IKKα and β.

Advanced biology·2025

Related Experiment Video

Updated: Oct 7, 2025

Preparation of DNA-crosslinked Polyacrylamide Hydrogels
09:06

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Published on: August 27, 2014

14.8K

Facile and Versatile Method for Micropatterning Poly(acrylamide) Hydrogels Using Photocleavable Comonomers.

Dimitris Missirlis1, Miguel Baños1, Felix Lussier1

  • 1Department of Cellular Biophysics, Max-Planck-Institute for Medical Research, Jahnstr. 29, Heidelberg 69120, Germany.

ACS Applied Materials & Interfaces
|January 10, 2022
PubMed
Summary

This study introduces a simple UV-based method to pattern hydrogels with molecules. This technique allows precise cell patterning and is compatible with traction force microscopy (TFM).

Keywords:
cell−material interactionsintegrin ligandsmechanotransductionphotopatterningtraction force microscopy

More Related Videos

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
11:31

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues

Published on: August 28, 2014

13.6K
Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning
12:07

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

13.6K

Related Experiment Videos

Last Updated: Oct 7, 2025

Preparation of DNA-crosslinked Polyacrylamide Hydrogels
09:06

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Published on: August 27, 2014

14.8K
Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
11:31

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues

Published on: August 28, 2014

13.6K
Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning
12:07

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

13.6K

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Cell Biology

Background:

  • Precise control over surface chemistry is crucial for understanding cell behavior.
  • Existing micropatterning techniques often require complex procedures or specialized equipment.

Purpose of the Study:

  • To develop an accessible micropatterning strategy for creating arbitrary patterns of small molecules and ligands on hydrogel surfaces.
  • To enable precise control over cell adhesion and response based on pattern shape and substrate properties.

Main Methods:

  • Co-polymerization of a caged amine (NVOC group) monomer into poly(acrylamide) hydrogels.
  • Localized deprotection of amines using mild UV light for subsequent functionalization.
  • Demonstration of cell patterning using various cell-adhesive ligands.

Main Results:

  • Successful generation of arbitrary micropatterns on hydrogels without photomasks.
  • Demonstrated cell patterning and observed cellular responses to pattern shape and substrate elasticity.
  • Compatibility with standard and reference-free traction force microscopy (TFM).

Conclusions:

  • The presented method offers an easy-to-use, versatile platform for hydrogel surface functionalization.
  • This technique facilitates advanced cell studies, including biomechanical analyses using TFM.
  • The approach supports the patterning of diverse low-molecular-weight ligands for tailored biological applications.