In Ovo Testing Method for Inhalants on a Chorio-Allantoic Membrane

Area of Science:

• Oncology
• Pharmacology
• Biomedical Engineering

Background:

• Solid tumors and metastasis require angiogenesis for growth, making anti-angiogenic therapies a key strategy in cancer treatment.
• Developing effective delivery methods for anti-angiogenic agents is crucial for successful cancer therapy.

Purpose of the Study:

• To evaluate the anti-angiogenic potential of curcumin liposomes delivered via inhalation.
• To validate the chorio-allantoic membrane (CAM) assay as a method for testing inhaled anti-angiogenic formulations.

Main Methods:

• Utilized an *in ovo* chick embryo model (CAM assay) to assess angiogenesis.
• Tested curcumin liposomes as a model inhaled formulation for pulmonary application.
• Determined the direct effect of the inhaled formulation on blood vessel formation.

Main Results:

• Curcumin liposomes exhibited a significant and excellent anti-angiogenic effect in the CAM assay.
• The *in ovo* testing method proved effective in precisely evaluating the impact of inhaled substances on angiogenesis.

Conclusions:

• Curcumin liposomes show promise as an inhaled anti-angiogenic agent for cancer therapy.
• The *in ovo* CAM assay is a suitable and precise method for testing inhaled anti-cancer formulations.