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Fibronectin and immune complexes in rheumatic diseases
K E Herbert1, J S Coppock, A M Griffiths
1Department of Rheumatology, St Bartholomew's Hospital, West Smithfield, London.
Annals of the Rheumatic Diseases
|October 1, 1987
Summary
Fibronectin is integral to immune complexes in rheumatic diseases, potentially influencing immune system functions. This study reveals fibronectin
Area of Science:
- Rheumatology
- Immunology
- Biochemistry
Background:
- Rheumatic diseases involve complex immune system dysregulation.
- The role of fibronectin in immune complex formation within rheumatic conditions requires clarification.
Purpose of the Study:
- To investigate the presence and significance of fibronectin within immune complexes in rheumatic diseases.
- To determine if fibronectin is a component of immune complexes independent of C1q.
Main Methods:
- Gel filtration chromatography and two-dimensional immunoelectrophoresis were used to analyze serum fibronectin.
- Polyacrylamide gel electrophoresis with immunoblotting analyzed fibronectin in synovial fluid.
- Quantification of fibronectin in serum and synovial fluid from patients with rheumatoid arthritis and osteoarthritis.
Main Results:
- Fibronectin was found in immune complexes formed in vitro without C1q.
- Complexed fibronectin, coeluting with IgA and C3, was detected in rheumatoid vasculitis serum but not normal serum.
- Rheumatoid arthritis patients showed higher levels of fibronectin in serum and synovial fluid compared to osteoarthritis patients.
Conclusions:
- Fibronectin is an integral component of immune complexes in rheumatic diseases.
- Fibronectin may play a pathogenic role by influencing immune system functions in connective tissue diseases.
- These findings highlight fibronectin's involvement in the pathogenesis of rheumatic conditions.