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Updated: Oct 7, 2025
Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
Published on: September 8, 2023
Aligner-Mediated Cleavage-Based Isothermal Amplification for SARS-CoV-2 RNA Detection
Chao Zhang1, Tingting Zheng1, Hongliang Fan2
1Institute of Molecular Medicine (IMM), Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
A new aligner-mediated cleavage-based strand displacement amplification (AMC-SDA) assay rapidly detects SARS-CoV-2 RNA in under 25 minutes. This simple, instrument-free method offers high accuracy for diagnosing COVID-19 and emerging infectious diseases.
Area of Science:
- Molecular Biology
- Virology
- Diagnostic Assays
Background:
- Rapid detection of SARS-CoV-2 RNA is crucial for controlling COVID-19 transmission.
- Existing diagnostic methods may require specialized equipment or longer turnaround times.
Purpose of the Study:
- To develop a simple, rapid, and versatile assay for SARS-CoV-2 RNA detection.
- To establish a universal platform for identifying emerging infectious diseases.
Main Methods:
- Utilized aligner-mediated cleavage-based strand displacement amplification (AMC-SDA).
- The assay requires no professional instruments and has no specific target sequence requirements.
- Achieved a limit of detection in the attomolar RNA concentration range.
Main Results:
- The entire amplification procedure was completed in less than 25 minutes.
- Demonstrated 100% diagnostic accuracy using pseudovirus in 10 simulated samples (5 positive, 5 negative).
Conclusions:
- The AMC-SDA assay provides a rapid and accurate method for SARS-CoV-2 RNA detection.
- This versatile platform holds potential for the swift diagnosis of emerging infectious diseases.
