Rapid Analysis of Biological Samples Using Monolithic Polymer-Based In-Tube Solid-Phase Microextraction with Direct...

Area of Science:

• Analytical Chemistry
• Separation Science
• Mass Spectrometry

Background:

• Online coupling of extraction and detection methods simplifies workflows.
• Solid-phase microextraction (SPME) is a versatile technique for sample preparation.
• Mass spectrometry (MS) offers high sensitivity and specificity for analyte detection.

Purpose of the Study:

• To develop an online in-tube solid-phase microextraction (SPME) coupled with mass spectrometry (MS).
• To utilize a monolithic SPME capillary as an electrospray emitter for simplified device and operation.
• To analyze non-steroid anti-inflammatory drugs in plasma and urine samples.

Main Methods:

• Online in-tube SPME-MS using a monolithic SPME capillary as an electrospray emitter.
• Development of a porous organic polymer with high surface area and suitable porous structure.
• Application for the analysis of non-steroid anti-inflammatory drugs in biological matrices.

Main Results:

• Achieved efficient extraction and ionization due to the porous organic polymer.
• Demonstrated a simplified analytical process with a total analysis time of approximately 12 minutes for 100 μL samples.
• Exhibited excellent extraction performance, good reusability, and no clogging events.
• Obtained a wide linear range (0.1-200 ng/mL) with good linearity (R² ≥ 0.99) and reproducibility (intra-day RSDs <3.00%, inter-day RSDs <6.29%).

Conclusions:

• The developed online in-tube SPME-MS method offers a simplified, efficient, and robust approach for analyzing drugs in biological samples.
• The monolithic SPME capillary functions effectively as an electrospray emitter, enhancing ionization efficiency and stability.
• This method provides a promising alternative for routine analysis requiring high throughput and accuracy.