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Updated: Oct 7, 2025

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Quantitative Proteomics Using Isobaric Labeling: A Practical Guide.

Xiulan Chen1, Yaping Sun1, Tingting Zhang1

  • 1Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100149, China.

Genomics, Proteomics & Bioinformatics
|January 10, 2022
PubMed
Summary
This summary is machine-generated.

Isobaric labeling technology is crucial for comparing protein expression but faces accuracy challenges. This review details isobaric mass tags, workflow precautions, and applications in proteomics, thermal proteome profiling, and proteogenomics.

Keywords:
Isobaric labelingMass spectrometryQuantitative proteomicsTMTiTRAQ

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Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Biotechnology

Background:

  • Relative proteomic quantification is vital for biological sample comparison.
  • Isobaric labeling technology offers multiplexing and flexibility but has accuracy and precision challenges.

Purpose of the Study:

  • To provide a comprehensive overview of isobaric labeling technology.
  • To discuss precautions for reliable quantification in large-scale proteomics.
  • To highlight applications in biological and clinical studies.

Main Methods:

  • Overview of different isobaric mass tags.
  • Discussion of advantages and disadvantages of isobaric labeling.
  • Guidance on workflow precautions for quantitative proteomics.

Main Results:

  • Isobaric labeling is a key tool in proteomics despite quantitative challenges.
  • Careful workflow management is essential for reliable results.
  • The technology has broad applications including thermal proteome profiling and proteogenomics.

Conclusions:

  • Isobaric labeling technology is a powerful tool in proteomics.
  • Addressing accuracy and precision issues is crucial for reliable quantification.
  • Applications span diverse biological and clinical research areas.