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Weight Change Alters the Small RNA Profile of Urinary Extracellular Vesicles in Obesity

Dughyun Choi1, Sewon Kim2,3, Jeyoung Woo2

  • 1Division of Endocrinology and Metabolism, Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, Republic of Korea.

Obesity Facts
|January 10, 2022
PubMed
Abstract

Insights

Obesity alters urinary extracellular vesicle (EV) RNA profiles, with weight loss normalizing these patterns. This study reveals distinct small RNA signatures in obese individuals and their changes post-bariatric surgery.

Area of Science:

  • Biochemistry
  • Genomics
  • Nephrology

Background:

  • Obesity is a major risk factor for chronic kidney disease.
  • Urinary extracellular vesicle (EV) RNA profiles vary across kidney diseases.
  • The impact of obesity on urinary EV RNA is not well understood.

Purpose of the Study:

  • To analyze small RNA profiles of urinary EVs in obese patients before and after weight reduction.
  • To compare urinary EV RNA profiles of obese patients with healthy volunteers (HVs).

Main Methods:

  • Recruited age-sex-matched obese patients and HVs.
  • Performed small RNA sequencing on urinary EVs.
  • Analyzed urinary EV RNA profiles 6 months after bariatric surgery.

Main Results:

  • Obese patients exhibited significantly different urinary EV RNA proportions (transfer RNA and microRNA) compared to HVs.
  • 1,343 small RNAs were differentially expressed in obese patients' urinary EVs.
  • Weight reduction modulated the expression of specific urinary EV small RNAs, correlating with clinical parameters.

Conclusions:

  • Obese patients possess distinct urinary EV small RNA profiles compared to healthy individuals.
  • Weight reduction significantly alters urinary EV small RNA profiles in obese patients.
Keywords:
Extracellular vesicleObesityRNASmall untranslated

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