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Polyurethane Foam Rafts Supported In Vitro Cultures of Rindera graeca Roots for Enhanced Production of Rinderol,
Mateusz Kawka1, Irena Bubko2, Mirosława Koronkiewicz3
1Department of Pharmaceutical Biology and Medicinal Plant Biotechnology, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, Poland.
International Journal of Molecular Sciences
|January 11, 2022
Summary
Immobilizing Rindera graeca roots on PUF rafts enhances in vitro production of rinderol, a novel anticancer furanonaphthoquinone. This method overcomes low yields and enables efficient product removal, showing promise for cancer therapy development.
Area of Science:
- Plant Biotechnology
- Natural Product Chemistry
- Cancer Research
Background:
- The Boraginaceae family is rich in naphthoquinone derivatives, including lipophilic pigments.
- Rinderol, a novel furanonaphthoquinone with anticancer properties, is found in Rindera graeca.
- Low compound content in plant-based production is a significant challenge.
Purpose of the Study:
- To develop an efficient in vitro production method for rinderol.
- To investigate the effect of root immobilization on rinderol yield.
- To evaluate the anticancer activity of rinderol.
Main Methods:
- Immobilization of Rindera graeca roots on polyurethane foam (PUF) rafts in in vitro cultures.
- Chromatographic analysis to quantify rinderol production.
- Assessment of rinderol's biological activity on HeLa cervical cancer cells.
Main Results:
- Immobilized R. graeca roots stably produced rinderol, with highest yields in transformed root lines after 42 days (222.98 ± 10.47 µg/flask).
- PUF rafts served as the primary site for rinderol accumulation.
- Rinderol demonstrated rapid induction of cell death in HeLa cells at low concentrations.
Conclusions:
- Root immobilization on PUF rafts is a successful strategy for enhanced rinderol production and in situ removal.
- Rinderol is a promising lipophilic furanonaphthoquinone with potential proapoptotic activity for cancer therapy.
