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Related Experiment Videos

Rapid decrease in lymphocyte adherence to high endothelial venules in lymph nodes deprived of afferent lymphatic

H R Hendriks1, A M Duijvestijn, G Kraal

  • 1Department of Cell Biology, Medical Faculty, Free University, Amsterdam, The Netherlands.

European Journal of Immunology
|December 1, 1987
PubMed
Summary

Occluding lymph flow to lymph nodes (LN) rapidly reduces lymphocyte binding to high endothelial venules (HEV). This occurs before HEV flattening, suggesting altered cell surface molecules are key to lymphocyte adherence.

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Area of Science:

  • Immunology
  • Cell Biology
  • Anatomy

Background:

  • Lymph node (LN) afferent lymph flow is crucial for maintaining high endothelial venule (HEV) structure and function.
  • Lymphocyte trafficking into LNs occurs across HEV walls.

Purpose of the Study:

  • To investigate the impact of afferent lymph flow occlusion on lymphocyte binding to rat LN HEV.
  • To determine the specificity of T and B lymphocyte adherence to HEV post-occlusion.

Main Methods:

  • In vitro lymphocyte-binding assay on rat LN HEV.
  • Occlusion of afferent lymph flow.
  • Morphometric analysis of HEV endothelium and bound lymphocytes.
  • T/B cell ratio analysis.

Main Results:

Related Experiment Videos

  • Lymphocyte binding to HEV significantly decreased 1-6 weeks after lymph flow occlusion.
  • Reduced HEV surface area and fewer lymphocytes per unit area contributed to diminished binding.
  • The T/B cell ratio remained unchanged despite reduced lymphocyte adherence.
  • Decreased lymphocyte binding preceded HEV endothelium flattening.

Conclusions:

  • Reduced lymphocyte binding to HEV is an early event following afferent lymph flow occlusion.
  • Loss of endothelial cell determinants involved in lymphocyte binding is likely responsible.
  • HEV cell height is secondary to adherence determinants for lymphocyte binding.