Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Superelastic Ti-Zr-Nb-Sn Thin Films Fabricated by Magnetron Sputtering: Biocompatibility and Bacterial Biofilm Formation Assessment for Orthopedic Applications.

Journal of biomedical materials research. Part A·2026
Same author

Silk Sericin Functionalized with Carboxy Drugs for Dermocosmetic Applications.

ACS applied bio materials·2026
Same author

Identification of the differential and synergic lipotoxic patterns of oleic acid, palmitic acid, and their mixture in 3D HepG2/C3A tissue using liver-on-chip technology.

Biotechnology progress·2025
Same author

<i>In situ</i> formation and culture of cell spheroids in a low-binding 3D-printed biochip.

Lab on a chip·2025
Same author

Photodynamic Therapy Using Pulsed-Laser Irradiation of Gold Nanoparticles for the Treatment of Cancers with High Basal Oxidative Stress.

Nano letters·2025
Same author

Inclusion of Magnesium- and Strontium-Enriched Bioactive Glass into Electrospun PCL Scaffolds for Tissue Regeneration.

Polymers·2025

Related Experiment Video

Updated: Oct 7, 2025

Bioinspired Soft Robot with Incorporated Microelectrodes
08:24

Bioinspired Soft Robot with Incorporated Microelectrodes

Published on: February 28, 2020

8.9K

Multiscale-Engineered Muscle Constructs: PEG Hydrogel Micro-Patterning on an Electrospun PCL Mat Functionalized with

Megane Beldjilali-Labro1, Rachid Jellali1, Alexander David Brown2

  • 1Centre de Recherche de Royallieu, Biomechanics & Bioengineering Laboratory, CNRS, Université de Technologie de Compiègne, 60203 Compiegne, France.

International Journal of Molecular Sciences
|January 11, 2022
PubMed
Summary

Researchers developed a novel multi-scale scaffold to support skeletal muscle tissue engineering. This engineered construct enhances myoblast differentiation and organization, showing promise for muscle regeneration applications.

Keywords:
electrospinningmusclemyotubeorganoidscaffoldtissue engineering

More Related Videos

Melt Electrospinning Writing of Three-dimensional Poly(&#949;-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications
12:28

Melt Electrospinning Writing of Three-dimensional Poly(ε-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications

Published on: December 23, 2017

15.3K
Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography
16:06

Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography

Published on: February 11, 2011

19.0K

Related Experiment Videos

Last Updated: Oct 7, 2025

Bioinspired Soft Robot with Incorporated Microelectrodes
08:24

Bioinspired Soft Robot with Incorporated Microelectrodes

Published on: February 28, 2020

8.9K
Melt Electrospinning Writing of Three-dimensional Poly(&#949;-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications
12:28

Melt Electrospinning Writing of Three-dimensional Poly(ε-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications

Published on: December 23, 2017

15.3K
Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography
16:06

Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography

Published on: February 11, 2011

19.0K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Engineered skeletal muscle constructs require specific material properties, including stiffness and geometry, for optimal cell differentiation.
  • Existing scaffolds often struggle to meet the complex micro-environmental needs of skeletal muscle cells.

Purpose of the Study:

  • To design and characterize a multi-scale scaffold for skeletal muscle tissue engineering.
  • To evaluate the scaffold's ability to support C2C12 skeletal myoblast differentiation.
  • To assess the scaffold's potential for electrical stimulation applications.

Main Methods:

  • Microfabrication of polyethylene glycol lines on electrospun poly(ε-caprolactone) nanofiber sheets.
  • Coating scaffolds with gold nanoparticles for potential electrical stimulation.
  • Culturing C2C12 skeletal myoblasts on scaffolds for seven days.
  • Quantifying cell differentiation via gene expression analysis and confocal microscopy for myotube alignment and length.

Main Results:

  • The multi-scale bio-construct exhibited tunable mechanical properties.
  • Scaffolds supported skeletal muscle cell differentiation through all stages.
  • Enhanced parallel orientation of myotubes was observed (variation < 15°).
  • Sustained myogenic differentiation and improved organization of reconstructed skeletal muscle were demonstrated.

Conclusions:

  • The developed multi-scale scaffold effectively supports and enhances skeletal muscle cell differentiation and organization.
  • The scaffold's tunable properties and potential for electrical stimulation make it suitable for mimicking physiological muscle functions.
  • This work advances the field of skeletal muscle tissue engineering with a promising biomaterial construct.