Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

9.2K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
9.2K
Labeling DNA Probes03:31

Labeling DNA Probes

8.5K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.5K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

13.7K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
13.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quinones operate as proton-collecting antennas in energy-transducing membranes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Maternal age modulates progeny social behavior via a small RNA-neuropeptide axis.

bioRxiv : the preprint server for biology·2026
Same author

Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking.

Science advances·2025
Same author

All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins.

Nature communications·2025
Same author

The chloroplast-targeted long noncoding RNA CHLORELLA mediates chloroplast functional transition across leaf ageing via anterograde signalling.

Nature plants·2025
Same author

Monitoring nucleoside metabolism in living cells with a nucleobase analogue <i>via</i> fluorescence lifetime imaging.

Chemical communications (Cambridge, England)·2025

Related Experiment Video

Updated: Oct 7, 2025

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
10:00

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Published on: March 24, 2014

78.2K

Fluorescent Probes for STED Optical Nanoscopy.

Sejoo Jeong1, Jerker Widengren2, Jong-Chan Lee1,3

  • 1Department of New Biology, Daegu Gyeongbuk Institute of Science & Technology, Daegu 42988, Korea.

Nanomaterials (Basel, Switzerland)
|January 11, 2022
PubMed
Summary
This summary is machine-generated.

This review details requirements for fluorescent probes used in STED nanoscopy, a super-resolution technique. It covers advancements in fluorescent proteins, organic dyes, and nanoparticles for improved imaging.

Keywords:
STEDfluorescent nanoparticlefluorescent probefluorescent proteinorganic dyesuper-resolution microscopy

More Related Videos

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

13.0K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.0K

Related Experiment Videos

Last Updated: Oct 7, 2025

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
10:00

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Published on: March 24, 2014

78.2K
Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

13.0K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.0K

Area of Science:

  • Biophysics
  • Optical Microscopy
  • Nanotechnology

Background:

  • Optical fluorescence microscopy advancements are linked to fluorescent probe development.
  • Super-resolution microscopy overcomes the Abbe diffraction limit using fluorescent probe properties.
  • STED nanoscopy requires probes with specific photophysical and photochemical characteristics.

Purpose of the Study:

  • To outline the requirements for fluorescent probes in STED nanoscopy.
  • To review recent progress in developing fluorescent probes for STED nanoscopy.
  • To analyze the strengths and limitations of current fluorescent probes.

Main Methods:

  • Literature review of fluorescent probe development for STED nanoscopy.
  • Analysis of photophysical and photochemical properties of probes.
  • Discussion of fluorescent proteins, organic dyes, and nanoparticles.

Main Results:

  • Identified key properties for ideal STED nanoscopy probes: high photostability, depletability, low excitability, and biocompatibility.
  • Reviewed progress in fluorescent proteins, organic dyes, and nanoparticles for STED.
  • Detailed analysis of probe strengths and limitations.

Conclusions:

  • Fluorescent probe development is crucial for STED nanoscopy.
  • Meeting specific photophysical and photochemical requirements enhances probe performance.
  • Ongoing research focuses on optimizing probes for advanced super-resolution imaging.